Modifying Enzymes
New England Biolabs, Inc. XhoI – 25000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. beta-Agarase I – 100 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
Sigma Aldrich Fine Chemicals Biosciences Choline Oxidase from Alcaligenes sp. lyophilized powder, ≥10 units/mg solid | 9028-67-5 | MFCD00130787 | 100un
Choline Oxidase from Alcaligenes sp. lyophilized powder, ≥10 units/mg solid | 9028-67-5 | MFCD00130787 | 100un
Enzyme Research Laboratories Inc Human Prekallikrein 1.0mg Vial
Our plama purified Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Purity is determined by SDS-PAGE.
New England Biolabs, Inc. Thermostable USER III Enzyme - 50 units
Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil. Thermostable USER III Enzyme is a mixture of Afu Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease IV. Afu UDG catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3’ and 5’ sides of the abasic site so that base-free deoxyribose is released leaving a 5’ deoxyribose phosphate (dRP) and a 3’-hydroxyl. Thermostable USER III Enzyme also has 3’-diesterase activity which can remove 3’ phosphate, 3’-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. Thermostable USER III Enzyme is optimally active at 65C.
Sigma Aldrich Fine Chemicals Biosciences Nuclease P1 from Penicillium citrinum, 54576-84-0, MFCD00131010, 1VL
Lyophilized powder, ≥200 units/mg protein (E1%/280, 3′-5′-Phosphodiesterase), Synonym: Endonuclease P1. Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5′ or 3′ end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
Sigma Aldrich Fine Chemicals Biosciences Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G
Proteinase from Aspergillus melleus Type XXIII, >=3 units/mg solid | 9001-92-7 | MFCD00132092 | 25G
Med Vet International MethylPREDnisolone Acitate Injection (depo-medrol), 40mg/mL, 10mL
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
Methylprednisolone is used to treat conditions such as arthritis, blood disorders, severe allergic reactions, certain cancers, eye conditions, skin/kidney/intestinal/lung diseases, and immune system disorders.
LifeSensors SUMO Protease 1 500 Units
LifeSensors' industry leading SUMO Protease 1 (Ulp1), a highly active and robust recombinant protease, cleaves SUMO from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short linear sequences, SUMO Protease 1 recognizes the tertiary structure of SUMO. As a result, SUMO Protease 1 will not cleave within the fused protein of interest. 1 unit will cleave >90 µg of hSUMO3-GFP in 1 hr at 37C.
PACIFIC BIOSCIENCES OF CALIFORNIA INC PacBio HiFi Prep Kit, 96 Samples, Whole Genome Sequencing, Automated
The Revio SPRQ HiFi prep kit 96 is a comprehensive kit for preparing sequence-ready SMRTbell libraries from up to 96 samples. Optimized for automated high-throughput whole genome sequencing in human, plant, and animal samples, it supports gDNA input from 500 ng to 2 μg per sample.
- Supports 24–96 SMRTbell libraries from up to 96 samples.
- Optimized for automated high-throughput whole genome sequencing.
- Compatible with liquid handler automation systems.
- Comprehensive workflow includes DNA size selection and library construction.
- Ideal for human, plant, and animal whole genome sequencing.
- SPRQ chemistry reduces required gDNA mass.
- Libraries stable for up to 1 month at 4°C or 6 months at -20°C.
Sigma Aldrich Fine Chemicals Biosciences Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | 171483-40-2 | MFCD00677269 | 1MG
Lysoganglioside-GM1 from bovine brain >=95%, lyophilized powder | Purity: >=95% | 171483-40-2 | MFCD00677269 | 1MG
ABclonal Technology T5 Exonuclease
T5 Exonuclease is a double-stranded DNA-specific exonuclease and single-stranded DNA endonuclease that degrades DNA in the 5′ ?to 3′ ?direction. T5 Exonuclease can digest nucleotides starting from the 5 end or at gaps and nicks in linear or circular dsDNA. The enzyme does not digest supercoiled dsDNA and exhibits endonuclease activity on ssDNA
Carnabio Usa Inc MST2(STK3)/5UG/FULL-LENGTH
MST2(STK3), Full-length, Wild type, Amino Acid 1-491, Q13188, Expressed in Insect (sf21); 5microg